Science Immunology

Supplementary Materials

The PDF file includes:

  • Fig. S1. Lung flow cytometry gating scheme for myeloid cells.
  • Fig. S2. Lung flow cytometry gating scheme for detection of M.tb.-infected cells with overlay of mEmerald+ populations.
  • Fig. S3. BAL and lung flow cytometry gating scheme for cell sorting.
  • Fig. S4. Bacterial burden at the time of AM cell sorting.
  • Fig. S5. Gene expression heatmaps of naïve, bystander, and M.tb.-infected AMs 24 hours after aerosol infection.
  • Fig. S6. Gating strategy for sorting of ΔRD1-infected AMs and bead-positive AMs.
  • Fig. S7. Gene expression of BMDMs after treatment with fluorescent beads.
  • Fig. S8. Gene expression heatmaps of M.tb.-infected AMs 0.5, 1, 2, 4, and 10 days after aerosol infection.
  • Fig. S9. AM response to in vitro M.tb. infection with and without GM-CSF treatment.
  • Fig. S10. AM response to in vitro M.tb. infection at 8, 12, and 24 hours after infection.
  • Fig. S11. Gene expression differences between naïve WT and Nrf2−/− AMs.
  • Fig. S12. Baseline lung conditions of WT and Nrf2−/− mice.
  • Fig. S13. Gene expression and protein confirmation of NRF2 knockout in AMs from Nrf2LysM, Nrf2CD11c, and Nrf2fl littermate controls; WT; and Nrf2−/− mice.
  • Fig. S14. Baseline lung conditions of Nrf2LysM, Nrf2CD11c, and Nrf2fl mice.
  • Fig. S15. Gene expression heatmaps of M.tb.-infected Nrf2LysM, Nrf2CD11c, Nrf2fl, WT, and Nrf2−/− AMs 24 hours after aerosol infection.
  • Fig. S16. Viability of bystander Nrf2LysM, Nrf2CD11c, and Nrf2fl AMs 10 days after high-dose aerosol infection.
  • Legends for tables S1 to S6

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Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). Summary table accounting for M.tb.-infected cells in the lung 1 to 14 days after high-dose mEmerald-H37Rv infection.
  • Table S2 (Microsoft Excel format). IPA and HOMER results tables.
  • Table S3 (Microsoft Excel format). RNA-Seq data for WT AMs.
  • Table S4 (Microsoft Excel format). RNA-Seq data for Nrf2−/− AMs.
  • Table S5 (Microsoft Excel format). RNA-Seq data for Nrf2fl, Nrf2LysM, and Nrf2CD11c AMs.
  • Table S6 (Microsoft Excel format). Raw data file.

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