Science Immunology

Supplementary Materials

The PDF file includes:

  • Fig. S1. Examination of the “anergic state” of total and Ins-tetramer+CD4+ T cells in the spleen and PLNs based on FR4+/CD73+ surface labeling of the CD44 and CD44++ populations.
  • Fig. S2. Violin plot representation of the top most up-regulated and differentially expressed genes in clusters 1 to 4.
  • Fig. S3. Direct IFN-γ and TNF-α staining in islet-infiltrating CD4+ T cells in three 9-week-old NOD mice.
  • Fig. S4. Direct detection of CD25+Foxp3+ cells in the spleen, PLNs, and islets of 10-week-old female NOD mice.
  • Fig. S5. Diabetes incidence in NOD and heterozygote NOD × MHC class II−/− female mice.
  • Fig. S6. Response of the Ins13–21-specific T cell hybridoma 11T3 to the Ins9–23 peptide presented by NOD and NODβ57D splenocytes.
  • Table S1. Summary of the various peptide constructs that were used to produce recombinant I-Ag7/peptide complexes in fly cells.
  • Table S2. Comparison of all CDR3β analyzed for the present study to their genomic segments.

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Other Supplementary Material for this manuscript includes the following:

  • Table S3. Excel spreadsheet with raw data.

Files in this Data Supplement: