Science Immunology

Supplementary Materials

The PDF file includes:

  • Fig. S1. Gating strategies for developing B cells.
  • Fig. S2. Cell cycle analysis of tet-Lin28b ABM pre-B and ImmB subsets.
  • Fig. S3. Determination of the minimal DOX treatment window during B cell maturation required for efficient CD5+ B-1 cell output from tet-Lin28b ABM.
  • Fig. S4. Barcode filtering and analysis strategy.
  • Fig. S5. Differentially expressed genes within top enriched Hallmark gene sets.
  • Fig. S6. Genetic evidence implicating Lin28b in the CD19/PI3K/c-Myc pathway.
  • Fig. S7. Transient tet-Lin28b expression of ABM progenitors promotes the output of functionally competent CD5+ B-1 cells.
  • Table S2. Antibodies used in this study.

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Other Supplementary Material for this manuscript includes the following:

  • Data file S1 (Microsoft Excel format). Table S1: Raw data supplement.
  • Data file S2 (.gz format). VDJseq PerC B-1 CD5 slices.
  • Data file S3 (Microsoft Excel format). DESeq2 normalized counts for adult tet-Lin28b and WT ImmB RNA-seq.
  • Data file S4 (Microsoft Excel format). DESeq2 normalized counts for neonatal Lin28b+/+, Lin28b−/− ImmB RNA-seq.

Files in this Data Supplement: