Science Immunology

Supplementary Materials

The PDF file includes:

  • Materials and Methods
  • Material used for whole-slide immunofluorescence
  • Antibody combinations used for multicolor immunofluorescence on tissue sections
  • Experimental details of CSR analysis, n-n analysis, and cell cluster analysis
  • Fig. S1. Flow cytometric analysis of intratumoral TAN.
  • Fig. S2. Clinical relevance of TAN density and LOX-1 expression.
  • Fig. S3. Interaction of T cells subsets with TAN.
  • Fig. S4. Quantification and clinical relevance of CD3-CD66b conjugation.
  • Fig. S5. Quantification of T cell effector function in tumor regions dominated by either T cells or CD66b+ cells.
  • Table S1. Univariate Cox regression analysis of OS for the LSCC cohort.
  • Table S2. Multivariate Cox proportional Hazard analysis of OS for the LSCC cohort.
  • Table S3. Univariate Cox regression analysis of OS for the OSCC cohort.
  • Table S4. Multivariate Cox proportional hazard analysis of OS for the OSCC cohort.
  • Legends for movies S1 to S4

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.mp4 format). Identification and localization of TAN and TIL in the 3D HNC tissue architecture.
  • Movie S2 (.mp4 format). Segmentation of immune cell compartments in the tumor microenvironment.
  • Movie S3 (.mp4 format). Spatial interaction of TAN and TIL in tumor tissue in situ.
  • Movie S4 (.mp4 format). Spatial deconvolution of GrzB expression in TIL.
  • Data S1. Raw data (Excel).

Files in this Data Supplement: