Science Immunology

Supplementary Materials

The PDF file includes:

  • Materials and Methods
  • Fig. S1. Schematic diagram of high-content screening with lentiviral shRNA library.
  • Fig. S2. Expression level of target genes in shRNA-treated PL16T and THP-1 cells.
  • Fig. S3. Expression level of NP viral protein in shRNA-treated PL16T and THP-1 cells.
  • Fig. S4. Purification of recombinant proteins.
  • Fig. S5. MxA is required for IL-18 secretion from infected PL16T cells upon IAV infection.
  • Fig. S6. AIM2 and NLRC4 are not required for IL-1β secretion in PL16T cells upon IAV infection.
  • Fig. S7. Expression level of MxA, NLRP3, and caspase-1 in respiratory epithelial cells and macrophages.
  • Fig. S8. Expression level of NP viral protein in MxA KD PL16T cells exogenously expressing NLRP3.
  • Fig. S9. Expression level of viral proteins in transfected PL16T cells.
  • Fig. S10. ASC oligomerization and proteolytic activation of caspase-1 in IAV-infected NHBE cells.
  • Fig. S11. Efficiency of bone marrow transplantation.
  • Fig. S12. Inflammasome activation by H5N1 or H7N7 NP transfection.
  • Reference (47)

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