Science Immunology

Supplementary Materials

The PDF file includes:

  • Fig. S1. AS dose, TGF-β, and IL-2 in AS-dependent in vitro induction of Foxp3+ T cells.
  • Fig. S2. In vitro induction of FOXP3 in human Tconv cells by AS.
  • Fig. S3. Inability of AS to induce Treg-specific DNA hypomethylation in vitro in Tconv cells.
  • Fig. S4. In vitro effects of AS on the proliferative activity and the phenotype of nTreg cells.
  • Fig. S5. Effects of AS on gene expression by T cells, B cells, and DCs.
  • Fig. S6. Identification of AS targets by chemical proteomics.
  • Fig. S7. Dominant–negative type impaired kinase activity of KD CDK8 or CDK19 and Foxp3 induction in CD4+ T cells by their retroviral expression.
  • Fig. S8. Generation of CDK8/19 KO EL4 cell lines.
  • Fig. S9. Retroviral expression of KD or WT CDK8 in CD4+ T cells.
  • Fig. S10. Effects of AS on serine phosphorylation of STAT5.
  • Fig. S11. Effects of AS on genome-wide STAT5 binding.
  • Fig. S12. Plasma concentration and body weight of mice treated with various doses of AS.
  • Fig. S13. Induction of pTreg cells by AS treatment and antigen immunization.
  • Fig. S14. RNA-seq analysis of AS-induced pTreg cells.
  • Fig. S15. IL-2 augments in vivo AS-mediated pTreg induction.
  • Fig. S16. Activation of DCs by AS.
  • Fig. S17. Suppression of OVA-induced DTH by AS.
  • Fig. S18. In vitro Foxp3-inducing effects of AS in the presence of various inhibitors.
  • Fig. S19. Inhibition of STAT5 by CDK8/19 in activated Tconv cells and their Foxp3 expression induced by CDK8/19 inhibition via activating STAT5.
  • Table S1. Kinase selectivity profiles of AS2863619 and AS3334366.
  • Table S2. List of antibodies.

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Other Supplementary Material for this manuscript includes the following:

  • Table S3 (Microsoft Excel format). Raw data.

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