Science Immunology

Supplementary Materials

The PDF file includes:

  • Fig. S1. Intranasal administration of HDM induces neutrophilic inflammation in the lungs and airways of mice.
  • Fig. S2. Intranasal administration of HDM induces an increase in neutrophil proteases in the lungs and airways of mice.
  • Fig. S3. Intraperitoneal administration of anti-Ly6G antibody, 1A8, systemically depletes neutrophils in HDM-treated mice.
  • Fig. S4. Intraperitoneal administration of anti-Ly6G antibody, 1A8, specifically depletes neutrophils.
  • Fig. S5. Neutrophil-depleted mice display augmented type 2 inflammation, epithelial remodeling, and airway resistance after 3 weeks of HDM exposure.
  • Fig. S6. Neutrophil-depleted mice display comparable airway hyperresponsiveness after 3 weeks of HDM exposure.
  • Fig. S7. Augmented TH2 cytokine levels in neutrophil-depleted mice administered HDM for 1 week.
  • Fig. S8. ILC2s are the predominant source of IL-13 after 1 week of HDM administration.
  • Fig. S9. Gating strategy for isolation of ILC2s and CD4+ T cells by FACS.
  • Fig. S10. Augmented IL-5 and IL-13 levels in lungs of neutrophil-depleted mice administered HDM for 1 week are primarily derived from ILC2s.
  • Fig. S11. ILC2s from neutrophil-depleted mice administered HDM for 1 week produce more IL-5 and IL-13 on a per-cell basis.
  • Fig. S12. HDM-exposed neutrophil-depleted mice exhibit augmented DC numbers and antigen presentation.
  • Fig. S13. Lung concentrations of classical monocyte chemokines and flow cytometry gating strategy to identify MDPs and CDPs.
  • Fig. S14. G-CSF is elevated in the serum of neutrophil-depleted mice administered HDM.
  • Fig. S15. Neutrophil-depleted mice administered HDM produce elevated levels of IL-17 and IL-23.
  • Fig. S16. Neutralization of IL-17 reverts the effects of neutrophil depletion in HDM-administered mice.
  • Fig. S17. G-CSF directly augments TH2 cytokine production from mouse ILC2s.
  • Fig. S18. Neutrophil depletion alters the IL-23–IL-17–G-CSF regulatory feedback pathway to exacerbate TH2 cytokine production and allergen sensitization.
  • Legend for table S1
  • Table S2. List of antibodies used for flow cytometry staining.

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Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). Raw data file.

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