Science Immunology

Supplementary Materials

The PDF file includes:

  • Materials and Methods
  • Fig. S1. Characterization of Clec10a in C57BL/6J and NC/Nga mice.
  • Fig. S2. Phenotypes of NC/Nga-Clec10ac.706T>C mice.
  • Fig. S3. Phenotypes of HDM-induced dermatitis in Clec10a-deficient mice.
  • Fig. S4. Characterization of WT and Clec10a−/− MPs.
  • Fig. S5. Generation of BMMPs transfected with WT or mutated (Y3F) Clec10a.
  • Fig. S6. Generation of Clec10a-CD3ζ reporter cells and Clec10a-Fc chimera.
  • Fig. S7. HDM stimulation of human Clec10a-CD3ζ reporter cells and knockdown efficiency of human ASGR1.

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Other Supplementary Material for this manuscript includes the following:

  • Table S1 (.pdf format). The stop-gain and frameshift mutations in NC/Nga mouse confirmed by Sanger sequence.
  • Table S2 (.pdf format). Lectin microarray analysis of Clec10a-L in HDM.
  • Table S3 (.pdf format). Glycan microarray analysis of Clec10a-Fc.
  • Table S4 (.pdf format). The primers for Sanger sequencing validation of stop-gain and frameshift mutations in the NC/Nga genome.
  • Table S5 (.pdf format). Lectins used for lectin microarray.
  • Table S6 (.pdf format). Glycans used for glycoconjugate microarray.
  • Table S7. Raw data file (Excel file).

Files in this Data Supplement: