Science Immunology

Supplementary Materials

The PDF file includes:

  • Materials and Methods
  • Fig. S1. Representative images after BMT.
  • Fig. S2. FACS plots demonstrating the gating strategy for Lgr5-GFPhigh ISCs and for Paneth cells.
  • Fig. S3. Allogeneic BMT decreases lineage tracing from ISCs.
  • Fig. S4. Coculture of activated T cells and intestinal organoids.
  • Fig. S5. Investigation of T cell effector pathways responsible for T cell–mediated intestinal organoid toxicity.
  • Fig. S6. Tamoxifen treatment does not impair organoid numbers.
  • Fig. S7. Epithelial lineage markers ex vivo and in vivo.
  • Fig. S8. Supplemental analyses of IFNγ-induced apoptosis in intestinal epithelium.
  • Fig. S9. Phenotyping of IFNγ+ cells in recipient intestinal mucosa after BMT.
  • Fig. S10. IFNγ induces ISC apoptosis in vivo.
  • Table S2. List of antibodies used.
  • References (6374)

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Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). Raw data used to generate all graphs that have n < 25.

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