Science Immunology

Supplementary Materials

The PDF file includes:

  • Methods
  • Fig. S1. Flow cytometric analysis of LysM-GCN2 cKO splenic macrophages and bone marrow precursors.
  • Fig. S2. Gating strategies for sorting mouse and human tumor associated macrophages and MDSCs.
  • Fig. S3. Increased proinflammatory signaling pathways in cytotoxic T cell and myeloid clusters of LysM–GCN2 cKO versus GCN2fl/fl tumors.
  • Fig. S4. Single cell RNA sequencing of mouse intratumoral-MDSCs.
  • Fig. S5. Altered metabolism in TAMs lacking GCN2 function.
  • Fig. S6. Altered metabolism MDSCs lacking GCN2 function.
  • Fig. S7. IPA of upstream regulators in PBMϕs.
  • Fig. S8. Landscape of EIF2AK4 (GCN2) gene expression and somatic alterations across 10,967 tumors from 32 cancer types in the TCGA PanCan study visualized by cBioPortal.org (10, 11).
  • Table S1. Differential expression of genes in whole B16 tumors from GCN2fl/fl versus LysM–GCN2 cKO mice shown in Fig. 2C heatmap.
  • Table S2. Differential expression of genes from GCN2fl/fl versus LysM–GCN2 cKO TAMs shown in Fig. 2C heatmap.
  • Table S3. Differential expression of genes from GCN2fl/fl versus LysM–GCN2 cKO MDSCs shown in Fig. 2C heatmap.
  • Table S4. Top 20 differentially expressed genes in B16 clusters shown in Fig. 3A heatmap.
  • Table S5. Differential gene expression between CD8 T cell clusters 1 and 2 shown in Fig. 3D heatmap.
  • Table S6. Identified ATF4 ChIP-seq chromosome features in uPBMϕs versus aaPBMϕs.
  • Table S7. List of differentially regulated genes and their functions in human melanoma TAMs versus resting, unpolarized macrophages (uMϕs) shown in Fig. 7D heatmap.
  • Table S8. List of genes that are down-regulated after GCN2 KD in human M2 macrophages.
  • Table S9. List of genes that are up-regulated after GCN2 KD in human aaMϕ.
  • References (5053)

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