Science Immunology

Supplementary Materials

The PDF file includes:

  • Methods
  • Fig. S1. Intestinal polyclonal IgA responses in co-caged BM chimeric mice and kinetics of RV infection in WT mice.
  • Fig. S2. Intestinal anticommensal and anti-RV IgA responses in the separately caged BM chimeric mice.
  • Fig. S3. Analysis of intestinal microbiome in separate versus co-caged Ltb+/+ and Ltb−/− mice.
  • Fig. S4. Anti-RV IgA response is mainly T cell dependent, and the MLN is the major initiation site of AID induction in response to RV infection.
  • Fig. S5. LTβR signaling during adulthood is dispensable for the induction and maintenance of an IgA response against RV.
  • Fig. S6. Immunofluorescence stains of the MLN from mice that received LTβR-Ig or control Ig at E14/E17.
  • Fig. S7. Analysis of GC B cells and Tfh cells in the MLN.
  • Fig. S8. PC analysis in the MLN at d8 after RV infection.
  • Fig. S9. Analysis of lymphoid stromal cells in the MLN.
  • Fig. S10. Activation status and gene expression of MLN stromal cells and the effects of CD4+ T cell depletion on the anti-RV IgA response.
  • Fig. S11. Analysis of lymphoid stromal cells in the inguinal LN and MLN, and anti-RV IgA responses in Ccl19-Cre Ltbrfl/fl mice.
  • Fig. S12. Schematic of proposed mechanisms for early-life LTβR signaling in regulating mucosal anti-RV IgA responses during adulthood.
  • References (90, 91)

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Other Supplementary Material for this manuscript includes the following:

  • Table S1. Raw data file (Excel spreadsheet).

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