Science Immunology

Supplementary Materials

The PDF file includes:

  • Materials and Methods
  • Fig. S1. CAR T cells induced tumor cells to enter pyroptosis.
  • Fig. S2. GSDME-mediated CAR T cell induced tumor cell pyroptosis.
  • Fig. S3. Perforin/granzyme B–caspase 3–GSDME pathway–mediated CAR T cell induced tumor cell pyroptosis.
  • Fig. S4. Tumor-specific CD8+ T cells induced tumor cells to enter apoptosis, not pyroptosis.
  • Fig. S5. High levels of perforin and granzyme B induce tumor cell pyroptosis.
  • Fig. S6. Tumor cell pyroptosis activated macrophages to release inflammatory cytokines.
  • Fig. S7. Pyroptotic tumor cells released ATP to activate macrophages.
  • Fig. S8. Tumor cell pyroptosis initiated CRS induced by CAR T cell therapy in vivo.
  • Fig. S9. ATP-Casp1-GSDMD pathway regulated the CRS induced by CAR T cell therapy in vivo.
  • Table S1. The general information from patients enrolled.
  • Table S2. Body temperature (ºC) from B-ALL patients after CD19 CAR T cell therapy.
  • Table S3. Serum level of IL-6 (pg/ml) from patients after CAR T cell therapy.
  • Table S4. Serum level of IFN-γ (pg/ml) from patients after CAR T cell therapy.
  • Table S5. Serum level of IL-10 (pg/ml) from patients after CAR T cell therapy.
  • Legends for movies S1 and S2

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.avi format). HER2+ m-Cherry–MCF-7 cells were cocultured with HER2-CAR T cells.
  • Movie S2 (.avi format). HER2+ m-Cherry–SGC-7901 cells were cocultured with HER2-CAR T cells.

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