Science Immunology

Supplementary Materials

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  • Methods
  • Fig. S1. Generation of TAs to mouse IgG2b.
  • Fig. S2. IgT depletion does not affect the survival rate of IgT-depleted fish.
  • Fig. S3. Ich-specific Ig responses in the serum from nondepleted and IgT-depleted fish after infection.
  • Fig. S4. Control probe staining of trout gill cryosections.
  • Fig. S5. Cytokines and AMPs with unchanged expression of gene transcripts in gill tissue from control and IgT-depleted fish.
  • Fig. S6. Cytokine expression in gill tissue from control and IgT-depleted fish upon in vivo treatment with TLR agonists.
  • Fig. S7. sIgT coats specific subset of bacteria with beneficial and pathogenic characteristics.
  • Fig. S8. IgT depletion results in mild gill dysbiosis 1 week after depletion.
  • Fig. S9. Levels of IgT+ B cells, IgT protein, sIgT-microbiota coating, gill microbial translocation, and tissue damage of IgT-depleted fish revert to those of control fish at 13 weeks after depletion treatment.
  • Fig. S10. IgT recovery at 13 weeks after depletion treatment is sufficient to restore microbiome composition.
  • Table S1. Alpha diversity metrics in gill sIgT-coated bacteria.
  • Table S2. Bacterial community composition of trout gill sIgT-coated bacteria at the order level (in percentage).
  • Table S3. Significant OTUs 1 week after IgT depletion in trout gill.
  • Table S4. Bacterial community composition in control and IgT-depleted trout gill at the order level (in percentage) 1 week after depletion.
  • Table S5. Bacterial community composition in control and IgT-depleted trout gill at the genus level (in percentage) 1 week after depletion.
  • Table S6. Bacterial community composition in control and IgT-depleted trout gill at the order level (in percentage) 3 weeks after depletion.
  • Table S7. Significant OTUs 3 weeks after IgT depletion in trout gill.
  • Table S8. Bacterial community composition in control and IgT-depleted trout gill at the genus level (in percentage) 3 weeks after depletion.
  • Table S9. Bacterial community composition in control and IgT-depleted trout gill at the phylum level (in percentage) 13 weeks after depletion.
  • Table S10. Significant OTUs 13 weeks after IgT depletion in trout gill.
  • Table S11. Bacterial community composition in control and IgT-depleted trout gill at the genus level (in percentage) 13 weeks after depletion.
  • Table S12. Primer sequences used for real-time PCR.
  • References (7178)

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