Science Immunology

Supplementary Materials

The PDF file includes:

  • Fig. S1. Characterization of BP3+ and BP3lo-neg FRCs.
  • Fig. S2. Characterization of lymph node AFC responses and localization after OVA-Alum.
  • Fig. S3. Characterization of immune cell populations in homeostatic and immunized Ccl2−/− lymph nodes and spleens.
  • Fig. S4. YFP expression in Ccl19-Cre;YFPf/STOP/f FRCs at day 15 after OVA-Alum.
  • Fig. S5. Transplanted lymph nodes show normal organization.
  • Fig. S6. Analysis of optimal transplants and immune responses.
  • Fig. S7. Further characterization of CCR2 expression and CCR2+ cell identity, numbers, and localization after immunization.
  • Fig. S8. Monocyte numbers are lower in homeostatic Ccl2−/− lymph nodes, and neutrophils do not play a role in limiting antibody responses.
  • Fig. S9. Effects of interferon receptor blockade on stromal CCL2, anti–VE-cadherin on cell populations and FRC phenotype, and serum on FRC CCL2.

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