Science Immunology

Supplementary Materials

The PDF file includes:

  • Fig. S1. Sorting of P14 CD8+ T cells from spleen and siIEL for scRNA-seq.
  • Fig. S2. Circulating and siIEL CD8+ T cells are transcriptionally distinct at all time points after infection.
  • Fig. S3. Differential gene expression of circulating and siIEL CD8+ T cells at day 4 after infection.
  • Fig. S4. Shared core transcriptional program among splenic and siIEL CD8+ T cells.
  • Fig. S5. Components of the TRM cellāˆ’enriched transcriptional signature.
  • Fig. S6. shRNA knockdown of putative regulators of TRM cell differentiation.
  • Fig. S7. PAGA trajectory analysis of TRM cell differentiation.

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Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). DE genes by splenic and siIEL CD8+ T cells at day 4 after infection.
  • Table S2 (Microsoft Excel format). Genes in Spleen and IEL Modules.
  • Table S3 (Microsoft Excel format). Genes in Combined Modules.
  • Table S4 (Microsoft Excel format). DE genes by day 60 versus day 90 TCM-like cells.
  • Table S5 (Microsoft Excel format). DE genes by Cluster 3 versus Cluster 29 cells.
  • Table S6 (Microsoft Excel format). DE genes by Cluster 17 versus Cluster 19 cells.
  • Table S7 (Microsoft Excel format). DE genes by Cluster 16 versus Cluster 20 cells.
  • Table S8. Raw data file (Excel spreadsheet).

Files in this Data Supplement: