Science Immunology

Supplementary Materials

The PDF file includes:

  • Fig. S1. Experimental design and gating strategy to isolate HDM-reactive memory TH and Treg cells.
  • Fig. S2. Expression of differentially expressed genes in HDM-reactive cells.
  • Fig. S3. Single-cell filtering of doublets and low HDM-reactive T cells.
  • Fig. S4. Single-cell clustering analysis using Seurat.
  • Fig. S5. Distribution of cell frequency for the seven HDM+ TH clusters for the 24 individuals.
  • Fig. S6. Coexpression of TH1- or TH17-specific signature genes.
  • Fig. S7. Donor and disease groups cell distribution in each of the HDM+ TH clusters.
  • Fig. S8. Coexpression of THIFNR-specific signature genes in publicly available dataset.
  • Fig. S9. Treg disease-related differences.
  • Fig. S10. Proportions of HDM-reactive Treg subsets among disease groups.
  • Fig. S11. TH2 single-cell analysis.

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Other Supplementary Material for this manuscript includes the following:

  • Table S1. Study participant details (Excel spreadsheet).
  • Table S2. Differential gene expression analysis in bulk populations (Excel spreadsheet).
  • Table S3. Single-cell analysis (Excel spreadsheet).
  • Table S4. Gene signature lists (Excel spreadsheet).
  • Table S5. Raw data file with statistics (Excel spreadsheet).

Files in this Data Supplement: