Science Immunology

Supplementary Materials

The PDF file includes:

  • Methods
  • Fig. S1. Representative ELISPOTs, frequencies of Env-specific ASCs/total IgG+ ASCs and LN LLPCs.
  • Fig. S2. Avidity scores and dissociation rate comparison across all groups at termination.
  • Fig. S3. Flow cytometry gating strategy in identifying LN GC B cells and TFH in Fig. 6.
  • Fig. S4. Whole LN images (at low magnification) for all animals euthanized ~4 weeks after the final vaccination in study #1.
  • Fig. S5. Correlation of LN GC B cells, TFH, LN, and BM PCs with nAbs at ~4 weeks after the final vaccination in study #1.
  • Fig. S6. Env-specific CD4+ T cell responses in study #1 by intracellular cytokine staining.
  • Fig. S7. Change in frequencies of CCR5+CD4+ T cells in rectal mucosa after vaccinations in study #1.
  • Fig. S8. Flow cytometry gating strategy in identifying monocyte subsets in fig. S9.
  • Fig. S9. Change in frequencies of monocyte subsets and activation markers after primary vaccination in study #1.
  • Fig. S10. Antibody responses (nAb, ADCC, and avidity) in study #2.
  • Fig. S11. Binding Ab responses against Env gp140, g120, V1V2, and gp41 by BAMA assay in study #2.
  • Fig. S12. Change in frequencies of monocyte subsets and activation markers after primary vaccination in study #2.
  • Table S1. Adjuvant characterization.
  • References (6675)

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