Science Immunology

Supplementary Materials

The PDF file includes:

  • Fig. S1. Replenishment kinetics of peritoneal and omental leukocytes in BM chimeric mice (associated with Fig. 1).
  • Fig. S2. Gating strategies and effects of OVX on peritoneal leukocytes (associated with Figs. 2 and 3).
  • Fig. S3. Effects of HFD on peritoneal macrophage replenishment kinetics.
  • Fig. S4. Strategy for the purification of CD102+ macrophages for RNA-seq analysis (associated with Fig. 4).
  • Fig. S5. Additional RNA-seq analysis.
  • Fig. S6. Sexual dimorphisms are present in mice from different housing environments and across strains (associated with Fig. 4).
  • Fig. S7. Certain sexual dimorphisms arise with age (associated with Figs. 5 and 6).
  • Fig. S8. Identification of leukocytes in peritoneal fluid during S. pneumoniae–induced peritonitis (associated with Fig. 7).

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Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). DEGs between male and female CD102+ peritoneal macrophages.
  • Table S2 (Microsoft Excel format). Pathway analysis from population-level RNA-seq of male and female CD102+ peritoneal macrophages.
  • Table S3 (Microsoft Excel format). Genes uniquely up-regulated by female CD102+ peritoneal macrophages compared with male peritoneal and male and female CD102+ pleural macrophages.
  • Table S4 (Microsoft Excel format). GSEA from population-level RNA-seq of male and female CD102+ peritoneal macrophages.
  • Table S5 (Microsoft Excel format). Cluster-defining genes from scRNA-seq.
  • Table S6 (Microsoft Excel format). Cluster-specific sexually dimorphic genes identified by scRNA-seq.
  • Table S7 (Microsoft Word format). Additional statistics for data in Fig. 5.
  • Table S8 (Microsoft Word format). List of antibodies used in this study.
  • Data file S1 (Microsoft Excel format). Raw data.

Files in this Data Supplement: