Science Immunology

Supplementary Materials

The PDF file includes:

  • Fig. S1. Chemical structures of conjugate vaccines.
  • Fig. S2. FACS gating strategy to detect memory CD8+ T cell populations.
  • Fig. S3. Glycolipid-peptide vaccination induces greater numbers of liver TRM cells than a relatively high dose of PR8-OVA.
  • Fig. S4. Conjugate vaccines lacking the FFRK cleavage sequence do not alter serum ALT levels.
  • Fig. S5. Processing of vaccines by cathepsin B.
  • Fig. S6. Generation of memory T cell responses to VOx.FFRK.NVFLP are partially dependent on CD40/CD40L interactions.
  • Fig. S7. Synthesis of Fmoc-VC-PAB-pNP.
  • Fig. S8. Synthesis of MaGC-PAB-CV-Fmoc.
  • Fig. S9. Synthesis of MaGC-PAB-CV-NH2.
  • Fig. S10. Synthesis of MaGC-PAB-CV-Non.
  • Fig. S11. Synthesis of V.FFRK.OVALP conjugates.
  • Fig. S12. Synthesis of SPAAC conjugate vaccine V.FFRK.NVYSP.
  • Fig. S13. Synthesis of SPAAC conjugate vaccine V.NVYLP.
  • Fig. S14. Synthesis of SPAAC conjugate vaccine V.FFRK.NVYLP.
  • Fig. S15. Synthesis of oxime conjugate vaccine VOx.FFRK.NVYSP.
  • Fig. S16. Synthesis of oxime conjugate vaccine VOx.FFRK.NVFLP.
  • Fig. S17. Nuclear magnetic resonance (NMR) Spectra of Fmoc-VC-PAB-pNP.
  • Fig. S18. NMR spectra of MaGC-PAB-CV-Fmoc.
  • Fig. S19. NMR spectra of MaGC-PAB-CV-NH2.
  • Fig. S20. NMR spectra of MaGC-PAB-CV-Non.
  • Fig. S21. High-performance liquid chromatography (HPLC) analysis of V.FFRK.OVALP C-24 vaccine.
  • Fig. S22. HPLC analysis of V.FFRK.OVALP C22 vaccine.
  • Fig. S23. HPLC analysis of V.FFRK.OVALP C20 vaccine.
  • Fig. S24. HPLC analysis of V.FFRK.OVALP C18 vaccine.
  • Fig. S25. HPLC analysis of V.FFRK.OVALP C8 vaccine.
  • Fig. S26. HPLC analysis of V.FFRK.OVALP C4 vaccine.
  • Fig. S27. HPLC analysis of V.FFRK.NVYSP vaccine.
  • Fig. S28. HPLC analysis of V.NVYLP vaccine.
  • Fig. S29. HPLC analysis of V.FFRK.NVYLP vaccine.
  • Fig. S30. HPLC analysis of VOx.FFRK.NVYSP vaccine.
  • Fig. S31. HPLC analysis of VOx.FFRK.NVFLP vaccine.

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.mp4 format). Imaging of NKT cells within the liver of na├»ve and vaccinated mice.
  • Movie S2 (.mp4 format). Imaging of PbT-I TRM cells within the liver of mice vaccinated with V.FFRK.NVYSP.
  • Table S1 (Microsoft Excel format). Raw data.

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