Science Immunology

Supplementary Materials

The PDF file includes:

  • Materials and Methods
  • Fig. S1. Defective WRC complex assembly and stability.
  • Fig. S2. T cell phenotype and function in human HEM1 deficiency.
  • Fig. S3. Extended B cell immunophenotyping in human HEM1 deficiency.
  • Fig. S4. Detailed characterization of Hem1−/− mice.
  • Fig. S5. Single-cell RNA-seq analyses of splenic T and B cell subclusters.
  • Fig. S6. Extended characterization of B cells and comparison with WASP-deficient B cells.
  • Table S2. List of discriminating features for PCA of healthy donor and HEM1-deficient T cells upon fibronectin stimulation.
  • Table S3. T cell immunophenotyping in HEM1-deficient individuals compared with age-matched reference values.
  • Table S4. List of discriminating features for PCA of healthy donor and HEM1-deficient B cells upon IgM stimulation.
  • Table S5. B cell immunophenotyping in HEM1-deficient individuals compared with age-matched reference values.
  • Table S6. List of top differentially regulated genes in B cell subclusters as identified by 10× single-cell sequencing.
  • Table S7. List of top differentially regulated genes in T cell subclusters as identified by 10× single-cell sequencing.
  • Table S8. GO term enrichment of HEM1-deficient B cell functions derived from the top candidates from table S6.
  • Table S9. GO term enrichment of HEM1-deficient T cell functions derived from the top candidates from table S7.
  • Full Western blot scans for all figures.
  • References (7680)

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Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). Figure raw data.

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