Science Immunology

Supplementary Materials

The PDF file includes:

  • Materials and Methods
  • Fig. S1. CXCL2-GFP reporter system used to identify CXCL2-expressing cells by flow cytometry.
  • Fig. S2. Involvement of CARD9 in CXCL2 expression by AMs upon Cn in vivo stimulation.
  • Fig. S3. Similar distribution of CXCL2 and CXCL2+ AMs in the lung.
  • Fig. S4. Ontogenic FM with Flt3 in AM subpopulations.
  • Fig. S5. Assessing impacts of age and sex of mice on ratio between CXCL2 and CXCL2+ AMs.
  • Fig. S6. Characterization of gene expression in CXCL2+ and CXCL2 AMs.
  • Fig. S7. Comparison of metabolic profiles and phagocytosis between CXCL2+ and CXCL2 AMs.
  • Fig. S8. Annotating scRNA-seq data.
  • Fig. S9. Gene expression analyses using scRNA-seq data.
  • Fig. S10. Some AMs are preloaded with Cxcl2 mRNA.
  • Fig. S11. Combined analysis of RNA-seq and ATAC-seq data.
  • Fig. S12. Assessment of C1q-deficient mice in Cn infection.
  • Fig. S13. Apoptosis and survival of CXCL2 and CXCL2+ AMs.
  • Fig. S14. Donor AM subpopulations reconstituted from recipient mice through PMT.
  • Table S1. Curated M1 and M2 signature gene set.
  • Table S2. Antibodies used for flow cytometry.
  • Table S3. Primer sequence for qPCR.
  • Legends for data files S1 and S2
  • References (4865)

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