Science Immunology

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  • Fig. S1. Ablation of SNS results in T cell–dependent increased rate of tumor growth in MethA fibrosarcoma and MC38 colon carcinoma tumor models.
  • Fig. S2. CT26, MethA, and MC38 tumor cells lack ARs and transporters and do not respond to adrenergic stimulation.
  • Fig. S3. Representative MDSC gating strategy from SNS-ablated and control-treated tumor-bearing mice.
  • Fig. S4. Myeloid cells from SNS-ablated mice suppress CD4+ T cell proliferation.
  • Fig. S5. Single-cell RNA sequencing analysis of CD3CD19 splenocytes from SNS-ablated or control tumor-bearing mice.
  • Fig. S6. Identification of neutrophil-like cells in the spleen of CT26 tumor–bearing mice.
  • Fig. S7. Characterization of clusters in non-NK cells from tumor-bearing mice by analysis of selected differentially expressed genes.
  • Fig. S8. Ablation of SNS alters splenic polymorphonuclear cells toward a more immature, suppressive phenotype in tumor-bearing mice.
  • Fig. S9. Analysis of CD11b+Ly6CintLy6G+ cell maturity by flow cytometry validates findings of single-cell RNA sequencing in spleen.
  • Fig. S10. Single-cell RNA sequencing analysis of CD3CD19 bone marrow cells from SNS-ablated and control tumor-naïve mice.
  • Fig. S11. Analysis of neutrophil development by calculation of RNA velocity of single cells from the bone marrow.
  • Fig. S12. Gating strategy of MDSCs generated in vitro.
  • Fig. S13. Time course of SNS effect on Treg phenotype.
  • Fig. S14. Gating strategy and total numbers of iTregs generated in the presence of S100A8/A9.
  • Fig. S15. Efficiency of anti–Gr-1 antibody treatment in SNS-ablated or control mice.
  • Fig. S16. Proposed model of SNS-mediated regulation of the accumulation of MDSCs and tumor immunity.
  • Legend for table S1

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Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). Raw data file.