Science Immunology

The PDF file includes:

  • Supplementary Materials and Methods
  • 2D-MP cell adhesion assay
  • Fig. S1. Brain MuPyV-specific CD4 T cells express TH1 and TFH phenotypes and functions.
  • Fig. S2. MuPyV-specific CD4 T cells in the spleen have similar TCR affinities irrespective of IL-21 production.
  • Fig. S3. IL-21 production by CD4 T cells in both brain and spleen is associated with a TFH transcriptome.
  • Fig. S4. CD8 T cells require IL-21 signaling to become brain-resident memory T cells.
  • Fig. S5. CD4 T cell help and IL-21 signaling drive the metabolic profile of MuPyV-specific CD8 T cells.
  • Fig. S6. Flow gating strategies for CD8 and CD4 T cells in the brain.

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Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). Raw data file.
  • Table S2 (Microsoft Excel format). RNA-seq gene list.