Science Immunology

The PDF file includes:

  • Fig. S1. Single-cell RNA-seq data of peripheral blood collected from NSTEMI patient 28 hours after onset of symptoms.
  • Fig. S2. Temporal characterization of mouse intracardiac neutrophil subsets after MI by single-cell RNA-seq.
  • Fig. S3. A subset of post-MI cardiac neutrophils express SiglecF.
  • Fig. S4. Integrated single-cell transcriptomes of WT, Ifnar−/−, anti-Ifnar antibody treated, and Irf3−/− cardiac cells on D4 after MI.
  • Fig. S5. Single-cell RNA-seq of murine macrophages within the steady-state heart.
  • Fig. S6. Intracardiac ISG+ mononuclear cells from D4 post-MI cluster into same subsets as ISG cells except for Hrt-M4 (Nrf2-induced).
  • Fig. S7. Integrated single-cell transcriptomes of WT and Nrf2−/− cardiac macrophages on D4 after MI.
  • Fig. S8. Irf3- and Nrf2-induced mononuclear cells associate with opposite poles of differentiation by pseudo-time trajectory analysis (Monocle).
  • Fig. S9. Integrated single-cell transcriptomes of WT and Irf3−/− bone marrow and blood leukocytes in healthy and infarcted mice.
  • Fig. S10. Single-cell RNA-seq of bone marrow myeloid cells from Tet2-deficient mice at steady state and after MI.

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Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Micosoft Excel format). DEGs of integrated cardiac neutrophils from D1, D2, and D4 after MI.
  • Table S2 (Micosoft Excel format). DEGs of integrated cardiac monocytes and macrophages from WT, Ifnar−/−, anti-Ifnar antibody treated, and Irf3−/− mice.
  • Table S3 (Micosoft Excel format). DEGs of integrated cardiac monocytes and macrophages from WT and Nrf2−/− mice.
  • Table S4 (Micosoft Excel format). DEGs of integrated pre- and post-infarcted WT and Irf3−/− bone marrow and blood leukocytes.
  • Table S5 (Micosoft Excel format). DEGs of integrated pre- and post-infarcted WT and Tet2−/− bone marrow leukocytes.
  • Table S6. Raw data file (Excel spreadsheet).