Science Immunology

The PDF file includes:

  • Fig. S1. Development and validation of manual colorimetric and automated chemiluminescent assays for monitoring RBD and spike trimers antibodies in serum or plasma.
  • Fig. S2. Correlations between antibody levels and day of symptom onset to sample collection.
  • Fig. S3. IgG and IgA responses to the Nucleocapsid antigen of SARS-CoV-2 in serum.
  • Fig. S4. Effect of heat versus detergent inactivation of saliva samples on the detection of anti-RBD antibodies in a manual, colorimetric ELISA.
  • Fig. S5. IgG and IgA levels against SARS-CoV-2 antigens in the saliva of cohort 1.
  • Fig. S6. Strategy for calculating an integrated score in antigen-specific saliva assay.
  • Fig. S7. Validation of manual colorimetric assays for monitoring RBD and spike trimers antibodies in saliva.
  • Fig. S8. Dilution curves of positive control ELISAs for serum assays.
  • Fig. S9. Distribution of the intensity values across serum assays for negative controls and samples from patients with COVID-19 infection.
  • Table S1. ROC statistics table for ELISAs conducted on serum/plasma derived samples.
  • Table S2. Testing effect of Triton-X treatment on saliva samples.
  • Table S3. ROC statistics table for ELISAs conducted on saliva samples.

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Other Supplementary Material for this manuscript includes the following:

  • Table S4. Raw data file (Excel spreadsheet).