Science Immunology

The PDF file includes:

  • Fig. S1. Characterization of CASTAT5-transduced CD4+ T cell survival in vitro and in vivo.
  • Fig. S2. The transcriptomic sequencing analysis of CASTAT5 and control CD4+ T cells.
  • Fig. S3. The ATAC-seq analysis of CASTAT5 and control CD4+ T cells.
  • Fig. S4. CASTAT5 CD4+ T cells exhibit an epigenetic landscape in concordance with gene and protein expression profiles.
  • Fig. S5. RNA-seq and ATAC-seq data are consistent with published STAT5A ChIP-seq data.
  • Fig. S6. Motif analysis results from HOMER analysis.
  • Fig. S7. TF footprinting analysis identifies key TFs.
  • Fig. S8. scRNA-seq feature plots of individual genes.
  • Fig. S9. Gene coexpression network analysis using WGCNA package.
  • Fig. S10. Identification of STAT5A H299R and S711F mutations in CASTAT5 CD4+ T cells in both bulk and scRNA-seq datasets.
  • Fig. S11. CASTAT5-transduced CAR T cells exert enhanced antitumor effects in a solid tumor model.
  • Fig. S12. Transduction of primary human T cells with CASTAT5.

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Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). List of differentially expressed genes between CASTAT5 and control CD4+ T cells.
  • Table S2 (Microsoft Excel format). List of differential ATAC-seq peaks between CASTAT5 and control CD4+ T cells.
  • Table S3 (Microsoft Excel format). List of marker genes for the six clusters identified by scRNA-seq of CASTAT5 and control CD4+ T cells.
  • Table S4. Raw data file (Excel spreadsheet).