Science Immunology

The PDF file includes:

  • Fig. S1. Validation of conditional KO lines, parasite infection models, and the effect of antibiotics on parasite clearance.
  • Fig. S2. Dysregulated Treg phenotype and enhanced worm clearance in Foxp3Cre Gata3fl/fl mice.
  • Fig. S3. Identification of IL-33-GFP–expressing myeloid cell types in spleen and lung.
  • Fig. S4. Characterization of costimulatory molecules and TLR ligand–induced cytokine production from DCs derived from CD11cCre versus CD11cCreIL-33fl/fl mice.
  • Fig. S5. Cytoplasmic IL-33–expressing cells in the vicinity of intestinal Foxp3+Treg and BMDC spontaneously secrete IL-33.
  • Fig. S6. DC-intrinsic IL-33 controls differential gene expression and surface expression of perforin-2 in CD103+ cDC.
  • Fig. S7. Generation of founder Mpeg1-deficient mice and DC–T cell culture controls.
  • Fig. S8. Proposed working model for an IL-33/perforin-2 axis.

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Other Supplementary Material for this manuscript includes the following:

  • Table S1. Ingenuity Pathway Analysis showing genes involved in cytokine transport pathways that are down-regulated in DCs lacking IL-33 as compared with IL-33–sufficient DCs (Excel spreadsheet).
  • Table S2. Ingenuity Pathway Analysis showing upstream transcriptional regulators down-regulated in DCs lacking IL-33 as compared with IL-33–sufficient DCs (Excel spreadsheet).
  • Table S3. Raw data file (Excel spreadsheet).