Science Immunology

The PDF file includes:

  • Fig. S1. Representative ILC staining strategy.
  • Fig. S2. Generation of ILCs from CD34+α4β7+ HSC progenitors.
  • Fig. S3. Surface expression of CD48, CD52, CD117, and α4β7 by peripheral blood– and tonsil-derived CD34+ HSCs.
  • Fig. S4. Gene expression profiling in four subsets of hematopoietic progenitors.
  • Fig. S5. RNA-seq and single-cell culture demonstrated in vitro differentiation of NK cells and ILCs from CD34+ HSCs.
  • Fig. S6. Similar expression of NK cell–associated markers in CD34+α4β7+CD48+- and CD34+α4β7+CD48-derived NK cells.
  • Fig. S7. Differentially activated pathways in two distinct ILC3 subsets.
  • Fig. S8. The effect of CD244 signaling in the development of ILCs.
  • Table S1. Top 10 differentially expressed genes for each cluster within the terminally differentiated CD34+ HSC-derived ILCs.
  • Table S2. Table showing differentially expressed genes for clusters 3 to 6 that are common to both terminally differentiated cells and CD34+α4β7+ hematopoietic progenitors.
  • Table S3. Description of samples used for scRNA-seq analysis.
  • Table S4. Lists and description of primer assays used in the study.
  • Table S5. Lists and description of antibodies used in the study.

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Other Supplementary Material for this manuscript includes the following: