Science Immunology

The PDF file includes:

  • Fig. S1. Secondary structure–based multiple alignment of OfGSDME with other gasdermin family members.
  • Fig. S2. A model of the three-dimensional structure of OfGSDME.
  • Fig. S3. Phylogenetic analysis of OfGSDME.
  • Fig. S4. Multiple sequence alignment of the GSDME from invertebrates.
  • Fig. S5. Cleavage of human GSDME and mouse GSDMD by human caspases.
  • Fig. S6. Preparation of active form of rOfCASP3 and examination of its activity.
  • Fig. S7. Viability of the cells expressing OfGSDME or OfGSDME NT or CT domain.
  • Fig. S8. Morphology of the cells expressing different forms of OfGSDME NT domain.
  • Fig. S9. OfCASP3 activation and OfGSDME cleavage in transfected HeLa cells.
  • Fig. S10. Representative flow cytometry images of Annexin V-FITC/PI–stained cells.
  • Fig. S11. Representative time-lapse images of HeLaCASP3-/GSDMD- cells expressing mCherry-tagged OfGSDME observed with a confocal microscope.
  • Fig. S12. Inhibition of CASP3 activity in corals during V. coralliilyticus infection.
  • Table S1. Gasdermins used in the bioinformatic analysis.
  • Table S2. Primers used in the present study.
  • Legends for movies S1 to S5

[Download PDF]

Other Supplementary Material for this manuscript includes the following:

  • Table S3 (Microsoft Excel format). Raw data file for all figure graphs.
  • Movie S1 (.mp4 format). OfGSDME-NTS induces pyroptosis of HeLa cells.
  • Movie S2 (.mp4 format). OfGSDME-NTL induces pyroptosis of HeLa cells.
  • Movie S3 (.mp4 format). OfGSDME alone is unable to induce cell death.
  • Movie S4 (.mp4 format). Coexpressing OfGSDME and OfCASP3 induces pyroptosis.
  • Movie S5 (.mp4 format). Annexin V/Sytox Green double staining of OfGSDME- and OfCASP3-expressing cells.