Science Immunology

The PDF file includes:

  • Fig. S1. Presence of CD62Lhi KLRG1lo IL-7Rαhi subsets of antigen-specific CD8+ T cell during the expansion phase of acute infections.
  • Fig. S2. Presence of CD62Lhi KLRG1lo IL-7Rαhi subsets of endogenous non-TCR transgenic LCMV-specific CD8+ T cells during the expansion phase of LCMV-Arm infection.
  • Fig. S3. Expansion-phase expression of CD62L in all four LCMV-specific CD8+ T cell subsets defined by KLRG1 and IL-7Rα expression.
  • Fig. S4. Transcriptional signatures and functional properties of CD62Lhi, IL-7Rahi, and KLRG1hi CD8+ T cell expansion-phase subsets.
  • Fig. S5. Proliferative history of P14 CD8+ T cells subsets under conditions of physiological and supraphysiological precursor numbers.
  • Fig. S6. Phenotype of donor P14 CD8+ T cells at d8 after rechallenge infection.
  • Fig. S7. Comparison of CD62Lhi subsets during the antigen-driven expansion phase and the memory phase.
  • Fig. S8. Expression of costimulatory and cytokine receptors by CD8+ T cell subsets in the expansion phase of acute infection.
  • Fig. S9. Impact of strength of signal on expansion-phase CD8+ T cell subsets.
  • Fig. S10. Impact of PD-1 deficiency on the CD62Lhi population based on route of acute infection.
  • Fig. S11. Combined PD-1 and LAG-3 deficiency compromises immunological recall responses and the generation of secondary CD8+ T cell memory.
  • Legends for tables S1 to S4

[Download PDF]

Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). DEGs in clusters from d7 LCMV-Arm scRNA-seq.
  • Table S2 (Microsoft Excel format). “Core” expression signatures of CD62Lhi, IL-7Rαhi, and KLRG1hi effector-phase CD8+ T cell subsets.
  • Table S3 (Microsoft Excel format). Gene expression signatures of CD62Lhi, IL-7Rαhi, and KLRG1hi effector-phase CD8+ T cell subsets using naive CD8+ T cells as the comparator.
  • Table S4 (Microsoft Excel format). Raw data table.