Science Immunology

The PDF file includes:

  • Materials and Methods
  • Fig. S1. Analysis of the Tabula Muris scRNA-seq BM dataset.
  • Fig. S2. E-cadherin is expressed in mouse peripheral blood and spleen basophils.
  • Fig. S3. Expression of specific markers in basophils, MCs, and their progenitor populations.
  • Fig. S4. RNA-seq shows that pro-BMPs are transcriptionally primed to the basophil and MC lineages.
  • Fig. S5. Pro-BMPs express type 2 cytokines and the transcription factor GATA-2, which regulates E-cadherin expression in the basophil lineage.
  • Fig. S6. Gating strategies for hematopoietic populations.
  • Fig. S7. Analysis of GMP differentiation in culture.
  • Legends for tables S1 to S6
  • References (6283)

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Other Supplementary Material for this manuscript includes the following:

  • Table S1. Differentially expressed genes (false discovery rate < 0.05) between pro-BMPs and DN-GMPs (RNA-seq); Excel spreadsheet.
  • Table S2. Differentially expressed genes (false discovery rate < 0.05) between pre-BMPs and DN-GMPs (RNA-seq); Excel spreadsheet.
  • Table S3. Differentially expressed genes (false discovery rate < 0.05) between basophils and DN-GMPs (RNA-seq); Excel spreadsheet.
  • Table S4. IPA of the upstream regulators of differentially expressed genes between pro-BMPs and DN-GMPs (related to table S1); Excel spreadsheet.
  • Table S5. Genes associated with the indicated lineages for the calculation of cell probability scores; Excel spreadsheet.
  • Table S6. Raw data file; Excel spreadsheet.