Science Immunology

The PDF file includes:

  • Methods
  • Fig. S1. Histocytometry analysis of myeloid cell positioning.
  • Fig. S2. Analysis of myeloid cell recruitment and localization within LNs in response to various stimuli.
  • Fig. S3. Chemokine receptors expressed by cDCs and validation of DC-CCR7.KO BMC system.
  • Fig. S4. HEV-mediated recruitment of MOs in the dLNs.
  • Fig. S5. Res cDC relocalization and antigen presentation in the regulation of CD8 T cell responses.
  • Fig. S6. IL-12 production by TZ-localized inflammatory MOs after immunization.
  • Fig. S7. Myeloid cell microenvironments in the generation of T cell responses.
  • Fig. S8. Regulation of T cell differentiation by inflammatory MOs.
  • Fig. S9. Model of innate and adaptive immune cell cross-talk during type I inflammation.
  • Legend for movie S1
  • Table S1. Description of cellular phenotypes for flow and imaging studies.

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.mp4 format). Two-photon intravital microscopy visualization of MO trafficking into dLNs via local blood vessels.
  • Table S2 (Microsoft Excel format). Raw data file.