Science Immunology

The PDF file includes:

• Supplementary Methods
• Fig. S1. In-depth characterization of nasal polyp MCs.
• Fig. S2. Characterization of nasal polyp MC protease phenotype and localization.
• Fig. S3. Characterization of sorted cells through scRNA-seq.
• Fig. S4. Immunohistologic localization of EBI2-expressing MCs.
• Fig. S5. Identification of MCs in three IPF scRNA-seq datasets.
• Fig. S6. Identification of an asthma-enriched CD38^high MC cluster.
• Fig. S7. Influence of dupilumab on nasal polyp MC transcript expression.
• Fig. S8. Schematic representation of strategy for evaluating the transcriptome of Itgβ7^high MCs.
• Fig. S9. Proposed model for MC homeostasis and hyperplasia in human sinus mucosa.
• Legends for tables S1 to S7

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Other Supplementary Material for this manuscript includes the following:

• Table S1 (.zip format). Patient information.
• Table S2 (Microsoft Excel format). Gene lists derived from literature and databases used for scoring.
• Table S3 (Microsoft Excel format). Cell-by-gene matrix (raw) for sorted MCs from six polyp donors.
• Table S4 (Microsoft Excel format). Cluster defining marker genes, MC subset defining genes, and differential expression full gene lists presented in manuscript.
• Table S5 (Microsoft Excel format). Cluster defining marker genes for scRNA-seq datasets from the human IPF lung atlas and asthma versus healthy donors.
• Table S6 (Microsoft Excel format). Differential gene expression analysis for human umbilical cord–derived MCs treated with IL-4 or vehicle for 96 hours.
• Table S7 (Microsoft Excel format). Source data for figures.