Science Immunology

The PDF file includes:

  • Fig. S1. Phage display yields human Fabs with high affinity and specificity for mouse erythrocytes.
  • Fig. S2. No significant differences between transcripts of naïve and OVAlow.
  • Fig. S3. Synthesis of an irrelevant-Fab-OVA to control for size and expression vector of antigen.
  • Fig. S4. A8B1 persists on erythrocytes and is capable of stimulating T cells for days after initial dosing.
  • Fig. S5. T cell dysfunction is not due to bolus of antigen presentation immediately after injection.
  • Fig. S6. Characterization of the Batf3-deficient mouse.
  • Fig. S7. Flow cytometry gating strategy for OT-I and OT-II cells.
  • Fig. S8. Flow cytometry gating strategy for inhibitory, exhaustion, and anergy receptors.
  • Fig. S9. Flow cytometry gating strategy for tetramer staining.

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Other Supplementary Material for this manuscript includes the following:

  • Table S1. Differentially expressed genes between A8B1, OVAhigh, OVAlow, and naïve (Excel file).
  • Table S2. Ingenuity pathway analysis of A8B1 versus OVAhigh (Excel file).
  • Table S3. Amino acid sequences for Fab and antigen (Excel file).
  • Table S4. List of antibodies for flow cytometry (Excel file).
  • Table S5. Raw data (Excel file).