Science Immunology

The PDF file includes:

  • Supplementary Materials and Methods
  • Fig. S1. KRAS neoantigen transitions detected through MANA-SRM.
  • Fig. S2. Design and sequencing of CDR-H3 of the phage library.
  • Fig. S3. Characterization of the V2 scFv.
  • Fig. S4. Characterization of the RAS Q61H, Q61L, and Q61R scFvs.
  • Fig. S5. Bispecific antibody format comparison.
  • Fig. S6. V2 scDbs made with various anti-CD3 clones.
  • Fig. S7. Specificity and stability of scDbs.
  • Fig. S8. Additional peptide-pulsing data.
  • Fig. S9. Western blots and cocultures with transfected COS-7 cells.
  • Fig. S10. HLA-A3 expression in target cell lines.
  • Fig. S11. Sanger sequencing of RAS alleles in CRISPR-modified cell lines.
  • Fig. S12. V2-U2 scDb–mediated T cell activation in cocultures with NCI-H441 isogenic cell lines.
  • Fig. S13. Polyfunctional T cell response elicited by V2-U scDb.
  • Fig. S14. KRAS neoantigen transitions detected through MANA-SRM for NCI-H358 variants.
  • Fig. S15. V2-U2 scDb–mediated T cell activation in cocultures with NCI-H358 isogenic cell lines.
  • Fig. S16. Effects of V2 scDbs on IFN-γ secretion from T cells in cocultures with HLA-A3+ cell lines.
  • Fig. S17. HLA-A1 expression in target cell lines.
  • Fig. S18. Polyfunctional T cell response elicited by L2-U scDb.
  • Fig. S19. Testing potential cross-reactive peptides.
  • Fig. S20. Positional scanning of target peptides that could potentially react with V2 or L2 scDb.
  • Fig. S21. V2-U and L2-U effects on tumor growth in mouse model systems.
  • Fig. S22. Body weights of NSG mice treated with scDbs.
  • Fig. S23. Diagram of scFv phage library phagemid.
  • Table S1. MANA-SRM quantification.
  • Table S2. Peptides for pHLA complexes.
  • Table S3. scFv sequences.
  • Table S4. Binding kinetics of clones V2 and L2 to their respective MANA pHLA.
  • Table S5. V2 bispecific antibody sequences.
  • Table S6. KRAS G12 BLAST peptides.
  • Table S7. Human peptides matching the V2-U binding motif.
  • Table S8. Human peptides matching the L2-U binding motif.
  • References (6071)

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Other Supplementary Material for this manuscript includes the following:

  • Table S9 (Microsoft Excel format). Raw data table.