Science Immunology

The PDF file includes:

  • Fig. S1. Gating strategy to identify graft-infiltrating CD8+ and OT-I cells.
  • Fig. S2. Transcriptomic comparison among OT-I populations.
  • Fig. S3. scRNA-seq analysis of graft polyclonal T cells—classification strategy.
  • Fig. S4. Quantitation of flow cytometry data from Fig. 4.
  • Fig. S5. Distribution of polyclonal CD8+ T cells in tissues and allografts of parabionts.
  • Fig. S6. Representative pathology in retransplanted kidneys.
  • Fig. S7. Proliferation and IFNγ production in graft OT-I and polyclonal CD8+ TRM.
  • Fig. S8. Intravital two-photon microscopy of F1.OVA kidney graft.
  • Fig. S9. Anti–PD-1 treatment does not enhance rejection.
  • Fig. S10. Flow cytometry sorting strategy of graft OT-I and polyclonal T cells for scRNA-seq.

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Other Supplementary Material for this manuscript includes the following:

  • Table S1. Canonical pathways in OT-I TRM (Excel spreadsheet).
  • Table S2. Common tissue residency associated genes (Excel spreadsheet).
  • Table S3. Canonical pathways associated with tissue residency (Excel spreadsheet).
  • Table S4. Antibody list (Excel spreadsheet).
  • Table S5. Raw data file (Excel spreadsheet).