Science Immunology

The PDF file includes:

• Fig. S1. Gating strategy to identify graft-infiltrating CD8⁺ and OT-I cells.
• Fig. S2. Transcriptomic comparison among OT-I populations.
• Fig. S3. scRNA-seq analysis of graft polyclonal T cells—classification strategy.
• Fig. S4. Quantitation of flow cytometry data from Fig. 4.
• Fig. S5. Distribution of polyclonal CD8⁺ T cells in tissues and allografts of parabionts.
• Fig. S6. Representative pathology in retransplanted kidneys.
• Fig. S7. Proliferation and IFNγ production in graft OT-I and polyclonal CD8⁺ T_RM.
• Fig. S8. Intravital two-photon microscopy of F1.OVA kidney graft.
• Fig. S9. Anti–PD-1 treatment does not enhance rejection.
• Fig. S10. Flow cytometry sorting strategy of graft OT-I and polyclonal T cells for scRNA-seq.

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Other Supplementary Material for this manuscript includes the following:

• Table S1. Canonical pathways in OT-I T_RM (Excel spreadsheet).
• Table S2. Common tissue residency associated genes (Excel spreadsheet).
• Table S3. Canonical pathways associated with tissue residency (Excel spreadsheet).
• Table S4. Antibody list (Excel spreadsheet).
• Table S5. Raw data file (Excel spreadsheet).