Science Immunology

The PDF file includes:

  • Fig. S1. Assessing the cytotoxic potential of anti-CD19 CAR4 and CAR8 T cells in vivo using a genetically encoded reporter for apoptosis.
  • Fig. S2. In vitro phenotyping of CAR4 and CAR8 T cells before adoptive cell transfer.
  • Fig. S3. Kinetics of CAR T cell accumulation in blood and lymphoid organs.
  • Fig. S4. CAR4 T cells are poorly effective at eliminating endogenous B cells in vivo.
  • Fig. S5. CAR4 T cells are poorly effective at inducing tumor cell killing in vitro.
  • Fig. S6. Tumor sensitivity to cytokine-induced cell death.
  • Fig. S7. Identification of tumor-infiltrating immune cells by single-cell RNA sequencing.
  • Fig. S8. CAR T cells promote host immune cell recruitment at the tumor site.
  • Fig. S9. CAR T cell therapy induces a broad mobilization of host immunity in a model of pro–B leukemia cells.
  • Fig. S10. Combining CAR4 and CAR8 T cells improves antitumor activity.
  • Fig. S11. WT CAR T cells produce IFN-γ at the tumor site in vivo.
  • Fig. S12. CAR T cell–derived IFN-γ is sufficient to promote antitumor activity.
  • Fig. S13. WT and IFN-γ–deficient CAR8 T cells exhibit similar killing activities before in vivo transfer.
  • Fig. S14. CAR T cell–derived IFN-γ promotes IL-12 expression by DCs in vitro.
  • Fig. S15. CAR T cell–mediated antitumor activity relies on cellular cross-talk within the TME.
  • Legends for movies S1 to S7

[Download PDF]

Other Supplementary Material for this manuscript includes the following:

  • Movie S1. Both CAR4 and CAR8 T cells infiltrate and patrol tumor-invaded bone marrow (.avi file).
  • Movie S2. Direct tumor cell killing mediated by a CAR4 T cell (.avi file).
  • Movie S3. Direct tumor cell killing mediated by a CAR8 T cell (.avi file).
  • Movie S4. Tumor B cell undergoing apoptosis without detectable contact with CAR4 T cells (.avi file).
  • Movie S5. Tumor B cell undergoing apoptosis without detectable contact with CAR8 T cells (.avi file).
  • Movie S6. WT CAR8 T cells induce efficient tumor cell killing in the bone marrow (.avi file).
  • Movie S7. IFN-γ–deficient CAR8 T cells are poorly cytotoxic at the tumor site (.avi file).
  • Table S1. Raw data file (Excel file).