Science Immunology

The PDF file includes:

  • Fig. S1. Enhanced stability of disulfide-linked RL9 HLA-E complexes versus conventionally refolded RL9-HLA-E material, illustrated by thermal melt analysis.
  • Fig. S2. Representative FACS plot of a RL9 clone stained with HLA-E-RL9 disulfide-linked tetramer and fixed without wash.
  • Fig. S3. Creation of the CD8+ Jurkat (J8) T cell line.
  • Fig. S4. Activation of primary CD8+ T cells transduced with RL9-responsive TCRs by HLA-E RL9 stimulation.
  • Table S1. CRISPR guides for TCR and CD4 deletion in Jurkat cells.

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Other Supplementary Material for this manuscript includes the following:

  • Table S2. Raw data (in Excel spreadsheet).