Science Immunology

The PDF file includes:

• Fig. S1. Functional pentameric complex analysis of 68-1.2–based RhCMV vectors.
• Fig. S2. Protein sequence comparison of RhCMV/HCMV orthologs RhCMV157.5/UL128, Rh157.4/UL130, and Rh158-161/UL146-147.
• Fig. S3. Genetic configuration of gene-modified dd RhCMV FL vectors.
• Fig. S4. Transcriptional analysis of Rh158-Rh161 region genes in RhCMV FL in comparison to RhCMV 68-1 and 68-1.2.
• Fig. S5. Analysis of SIV-specific T cell responses in BAL in RM cohorts destined for SIV challenge.
• Fig. S6. Epitope analysis of RhCMV vaccine–elicited, SIV-specific CD8⁺ T cell responses in RM cohorts destined for SIV challenge.
• Fig. S7. Analysis of SIVgag-specific CD4⁺ and CD8⁺ T cell response magnitude in tissues of 68-1 versus 68-1.2 RhCMV/SIVgag vector–vaccinated RMs at necropsy.
• Fig. S8. General gating hierarchy for ICS analysis.
• Table S1. Epitope analysis summary of all study RMs.
• Table S2. Primer/probe sets used for expression analysis of RhCMV-encoded inhibitors of unconventionally restricted CD8⁺ T cell priming.

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Other Supplementary Material for this manuscript includes the following:

• Table S3. RhCMV vector genetic modifications.
• Table S4. Raw data file: Nonquantitative epitope analysis data.
• Table S5. Raw data file: Quantitative data.