Science Immunology

The PDF file includes:

  • Fig. S1. Expression of DC-SCRIPT in the spleen and LN.
  • Fig. S2. Expression of DC-SCRIPT in BM progenitors.
  • Fig. S3. Increased pDC development potential in the absence of DC-SCRIPT.
  • Fig. S4. The influence of DC-SCRIPT deficiency on the development of DC-SCRIPT–expressing cell types.
  • Fig. S5. Normal lymphocyte development in the absence of DC-SCRIPT.
  • Fig. S6. Increased expression of pDC signature genes in DC-SCRIPT–deficient cDC1.
  • Fig. S7. Genome-wide analysis of DC-SCRIPT DNA binding.
  • Fig. S8. Genome-wide analysis of DC-SCRIPT DNA binding shows correlation with BATF3 and IRF8 binding.
  • Fig. S9. Normal antigen presentation in DC-SCRIPT–deficient cDC1s and cDC2s in response to peptide antigen.
  • Fig. S10. DC-SCRIPT specifically regulates IL-12p40 production in cDC1s.
  • Fig. S11. DC-SCRIPT–driven IL-12p40 production in cDC1s is required for an optimal immune response.
  • Table S6. Antibodies used in this study.
  • Table S7. Primer sequences.

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Other Supplementary Material for this manuscript includes the following:

  • Table S1. Differentially expressed genes in cDC1s (.xls file).
  • Table S2. Differentially expressed genes in cDC2s (.xls file).
  • Table S3. Top 100 differentially expressed genes between WT cDC1 and cDC2 (.xls file).
  • Table S4. DC-SCRIPT peaks called in cDC1 and cDC2 (.xls file).
  • Table S5. Transcription factor–cobound regions in cDC1 and cDC2 (.xls file).
  • Table S8. Raw data table (.xls file).