Science Immunology

The PDF file includes:

  • Fig. S1. Gating strategies for sorting HLA-DRB1*04:01Fibβ-74cit69-81–specific CD4+ T cells.
  • Fig. S2. Characterization of HLA-DRB1*04: 01Fibβ-74cit69-81–specific CD4+ TCR clonotype usage in naïve and immune HLA-DR4 transgenic mice.
  • Fig. S3. Characterization of HLA-DRB1*04:01Fibβ-74cit69-81–specific CD4+ TCR repertoire in naïve and immune HLA-DR4 transgenic mice.
  • Fig. S4. Overlaid interactions of M134 and M141 TCR–HLA-DRB1*04:01Fibβ-74cit69-81 complexes.
  • Fig. S5. Affinity analysis of M134 TCR point mutants for HLA-DRB1*04:01Fibβ-74cit69-81.
  • Fig. S6. Representative TCR binding curves determined by SPR across HLA-DRB1*04:01 shared epitope mutant.
  • Fig. S7. Representative TCR binding curves determined by SPR across HLA-DRB1*04:05 allomorph.
  • Fig. S8. Overview structure of TCR-DR04:01Fibβ-72,74cit complexes.
  • Table S1. List of HLA-DRB1*04:01Fibβ-74cit69-81–specific CD4+ T cells in naïve and immune HLA-DR4 transgenic mice.
  • Table S2. Data collection and refinement statistics of TCR peptide–HLA-DR4 structures.
  • Table S3. TCR–pMHC II complex statistics.
  • Table S4. Contacts of G08 TCR–HLA-DRB1*04:01Fibβ-74cit69-81.
  • Table S5. Contacts of M134 TCR–HLA-DRB1*04:01Fibβ-74cit69-81.
  • Table S6. Contacts of M141 TCR–HLA-DRB1*04:01Fibβ-74cit69-81.
  • Table S7. Contacts of M134 TCR–HLA-DRB1*04:01Fibβ-72,74cit69-81.
  • Table S8. Contacts of M141 TCR–HLA-DRB1*04:01Fibβ-72,74cit69-81.
  • Table S9. Contacts of G08 TCR–HLA-DRB1*04:01Fibβ-72,74cit69-81.

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Other Supplementary Material for this manuscript includes the following:

  • Table S10. Raw data file (Excel spreadsheet).