Science Immunology

The PDF file includes:

  • Fig. S1. Susceptibility of Il1r1 WT and HET mice to C. rodentium and differential regulation of cytokines in Il1r1 HET and KO mice after C. rodentium infection.
  • Fig. S2. Contribution of IL-1α and IL-1β to host resistance against C. rodentium infection and activation of colon mesenchymal cells by IL-1α and IL-1β.
  • Fig. S3. Effect of recombinant RSPO3 on Lgr5 expression and crypt density in C. rodentium–infected Il1r1KO−/− mice.
  • Fig. S4. ISH of Rspo3 in uninfected and C. rodentium infected mice.
  • Fig. S5. Grem1 and Pdgfra coexpression and gating strategy for isolating GREM1+ and PDGFRα+ mesenchymal cells.
  • Fig. S6. Workflow of scRNA-seq analysis of GREM1+ and PDGFRA+ cell populations in human colonic biopsies from healthy control and ulcerative colitis diagnosed donors.
  • Fig. S7. Overview of different Cre lines used in our studies.
  • Fig. S8. Characterization of Il1r1 KO, Col1aWT/WT, Col1a1iIl1r1, PdgfraWT/WT, PdgfraiIl1r1, Grem1WT/WT, and Grem1iIl1r1 mice.
  • Fig. S9. IL-1R1–dependent increase in GREM1+ cells.
  • Fig. S10. Gating strategy for evaluating Col1a1 expression in ILCs and mesenchymal cells.
  • Fig. S11. Colon hyperplasia in Il1r1 WT and KO mice.
  • Fig. S12. Expression of Hopx in the colon of naïve mice and after DSS treatment.
  • Table S1. Sequences of qPCR primers and probes.
  • Table S2. Catalog numbers of ISH probes.
  • Table S3. Catalog numbers of antibodies.

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Other Supplementary Material for this manuscript includes the following:

  • Table S4. Raw data file (Excel spreadsheet).