Science Immunology

The PDF file includes:

  • Fig. S1. Quantification of TFH, TFR, and Treg cells from the three tissues used in the study.
  • Fig. S2. Characteristics of the cell populations from the three tissues used in the study.
  • Fig. S3. Heterogeneity of the single-cell transcriptomes from the different donors.
  • Fig. S4. Maturation stages of TFH cells.
  • Fig. S5. Proportion of Foxp3+ cells within different gates of tonsil.
  • Fig. S6. Assignment of bona fide TFR cells with comparison of TFH and TFR populations.
  • Fig. S7. Assignment of bona fide TFR cells with comparison of Treg and TFR populations.
  • Fig. S8. Flow cytometry profile of cells assigned as TFH, TFR, and IL10 TF.
  • Fig. S9. Protein validation by flow cytometry.
  • Fig. S10. Validation of the assignment of bona fide TFR cells with a public dataset from tonsil TFH cells.
  • Fig. S11. Treg heterogeneity between blood and tonsil.
  • Fig. S12. The top LVs.
  • Fig. S13. Calculated RNA velocity of a selection of genes.

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Other Supplementary Material for this manuscript includes the following:

  • Table S1 (.csv format). Differential scRNA-seq gene expression table for mature and immature clusters of all cells (text file).
  • Table S2 (.csv format). Differential scRNA-seq gene expression table for mature and immature clusters of TFH cells (text file).
  • Table S3 (.csv format). Differential scRNA-seq gene expression table for identifying bonafide TFR in sorted TFH and TFR cells (text file).
  • Table S4 (.csv format). Differential scRNA-seq gene expression table for identifying bonafide TFR in sorted TFR and Treg cells (text file).
  • Table S5 (.csv format). Differential scRNA-seq gene expression table for tissue TFH, TFR, and IL10 TF cells (text file).
  • Table S6 (.csv format). Differential scRNA-seq gene expression table for blood and tissue TFR cells. (text file).
  • Table S7 (.csv format). Differential scRNA-seq gene expression table for blood and tissue Treg cells. (text file).
  • Table S8 (Microsoft Excel format). Raw data file (Excel spreadsheet).