Table 2 Human IgG pharmacokinetic parameters of CHKV-24 in macaques after delivery of antibody-encoding mRNA.

The CHKV-24 NHP serum samples from study 2 were analyzed using the Human Therapeutic IgG1 ELISA Kit, with serum dilutions ranging from 1:100 to 1:1000. Parameters were calculated using Phoenix pharmacokinetic software (Certara, USA). A standard curve of absorbance at 450 nm versus log (concentration) was fit with a 4-parameter logistic equation for IgG1 quantification.

Tmax (hours)Cmax (μg/ml)AUC0–720 hour (hour*μg/ml)t1/2 (hours)
MeanSDCV%MeanSDCV%MeanSDCV%MeanSDCV%
240010.15.36533720195052.456165.811.7