The CHKV-24 NHP serum samples from study 2 were analyzed using the Human Therapeutic IgG1 ELISA Kit, with serum dilutions ranging from 1:100 to 1:1000. Parameters were calculated using Phoenix pharmacokinetic software (Certara, USA). A standard curve of absorbance at 450 nm versus log (concentration) was fit with a 4-parameter logistic equation for IgG1 quantification.
| Tmax (hours) | Cmax (μg/ml) | AUC0–720 hour (hour*μg/ml) | t1/2 (hours) | ||||||||
| Mean | SD | CV% | Mean | SD | CV% | Mean | SD | CV% | Mean | SD | CV% |
| 24 | 0 | 0 | 10.1 | 5.36 | 53 | 3720 | 1950 | 52.4 | 561 | 65.8 | 11.7 |